|
This test is based on a mixture of antibodies
raised against salbutamol and clenbuterol.
This "generic" test detects, due to the
antibody mixture, more Beta-agonists than
the Clenbuterol-EIA. The test is recommended
for the detection of the following
Beta-Agonists (% of cross-reactivity):
salbutamol (100%), clenbuterol (100%),
bromobuterol (100%), cimbuterol (75%),
mapenterol (70%), mabuterol (60%),
tulobuterol (50%), clenpenterol (50%),
clenproperol (50%), terbutaline (40%),
carbuterol (40%), cimaterol (10%) and
zilpaterol (1%). The dose-response curve
shows a measuring range for salbutamol of
0.06-2 ng/ml. Urine can be used directly in
this test after pH adjustment or, to lower
background, after an extraction. For faeces,
feed, tissue, milk, plasma, bile and
choroid/retina samples, simple extraction
methods are described in the manual. The
sample preparation procedures include an
enzymatic hydrolysis to guarantee valid
measurements of conjugated (glucuronidated /
sulphated) Beta-agonists like salbutamol,
terbutaline and carbuterol. Detection limits
as low as 0.1 ng/g can be reached depending
on the Beta-agonist, sample material and
clean-up procedure. |