19-Nortestosterone (also named nandrolone) belongs to the group of anabolic androgenic steroids. In some cases 19-nortestosterone is a naturally occurring compound (male pigs, pregnant cows). The use of growth promoters (e.g. anabolics) in meat production is banned in EC since 1986. In the 19-Nortestosterone EIA an antiserum is used showing cross-reactivities with the following compounds: 17ß-19-nortestosterone (100%), 17a-19-nortesterone (50%), nortestosterone-sulphate (100%), 19-nor-4-androstene-3,17-dione (240%), norgestrel (16%) and norethindrone (38%). The dose-response curve shows a measuring range for 17ß-19-nortestosterone of 0.06-2.0 ng/ml. Large amounts of urine samples can be screened in a short time for the presence of 17ß-19-nortestosterone and its related compounds. To reduce the influence of other urine compounds a solid phase extraction (C-18 Empore Extraction disk Cartridges) is recommended. Detection limits down to 0.5 ng/ml can be reached depending on the sample preparation procedure used.