Deoxynivalenol (DON) belongs to the trichothecenes, a family of closely related compounds produced by several Fusarium species. DON is also called vomitoxin, because intake of food contaminated with DON can result in vomit and nausea. The immunosuppressive activities are the other negative properties of the intake of DON contaminated food. In Europe, it is strongly advised to maintain the Maximum Levels for DON at 500 ppb for food and at 750 ppb for food and food components that still have to be processed. The DON ELISA can be applied for screening on the presence of DON in cereals, food, feed and silages. In this ELISA an antiserum is used that does not show cross-reactivity with 15-acetyl-DON and only for 97% cross-reacts with 3-acetyl-DON. This means that the overestimation of the DON concentration in a sample is 10% maximally as the 3-acetyl-DON concentration in a sample is not higher than 10%. For most matrices a detection limit down to 50 ppb can be reached. For samples of beer a detection limit down to 10 ppb can be reached.