Zearalenone is an oestrogenic compound, also known as F-2 mycotoxin, which occurs mainly in corn, sorghum, wheat, barley but also in banana’s, bean leaves, etc. Zearalenone is produced by the moulds Fusarium roseum, F. culmorum, F. moniliforme and F. graminiarum. The antibody used in this test cross-reacts with zearalenone and its main metabolites, but also with zeranol and its main metabolites. However, zeranol as anabolic compound does not occur in cereals, etc. The Zearalenone EIA can be used in combination with a fast and simple extraction procedure, or after a solid phase extraction method.