Chloramphenicol is a broad spectrum antibiotic which was widely used in veterinary medicine. Since 1994 the use of chloramphenicol is banned within the European Community. The Chloramphenicol EIA test is a competitive enzyme immunoassay for the screening of chloramphenicol in tissue, plasma, serum, urine, eggs, milk, honey and aquaculture products. Since the antibody used in this test system also recognizes chloramphenicol-glucuronide, this substance can be analyzed without prior hydrolysis. The dose-response curve shows a measuring range of 0.025 – 2 ng/ml. Diluted urine and defatted milk samples can be applied directly in the test with a detection limit of 0.1 ng/ml. After extraction of the sample using ethyl acetate detection limits of 0.01 ng/ml (urine, milk) and of 0.02 ng/g (egg, shrimp, muscle tissue, honey) were obtained. Using a ethyl acetate extraction for poultry feed and pork feed resulted in detection limits of 0.3 ng/g.