This test is based on a mixture of antibodies raised against salbutamol and clenbuterol. This “generic” test detects, due to the antibody mixture, more Beta-agonists than the Clenbuterol-EIA. The test is recommended for the detection of the following Beta-Agonists (% of cross-reactivity): salbutamol (100%), clenbuterol (100%), bromobuterol (100%), cimbuterol (75%), mapenterol (70%), mabuterol (60%), tulobuterol (50%), clenpenterol (50%), clenproperol (50%), terbutaline (40%), carbuterol (40%), cimaterol (10%) and zilpaterol (1%). The dose-response curve shows a measuring range for salbutamol of 0.06-2 ng/ml. Urine can be used directly in this test after pH adjustment or, to lower background, after an extraction. For faeces, feed, tissue, milk, plasma, bile and choroid/retina samples, simple extraction methods are described in the manual. The sample preparation procedures include an enzymatic hydrolysis to guarantee valid measurements of conjugated (glucuronidated / sulphated) Beta-agonists like salbutamol, terbutaline and carbuterol. Detection limits as low as 0.1 ng/g can be reached depending on the Beta-agonist, sample material and clean-up procedure.